huvec cell specific medium Search Results


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Millipore endogro huvecs
( A ) Gene sets involved in respiratory metabolism and enriched in KIR pos NK cells from NR and retrieved from GO. ( B ) Summary statistical graph showing the percentage of proliferating Ki67 pos /KIR pos NK cells (%, mean ± SD) in NR and R at 7–12 months after h-HSCT. ( C ) Summary statistical graph showing the percentage of KIR pos /NKG2C pos /IFN-γ pos NK cells in NR ( n = 3) and R ( n = 3) recipients at 8–12 months after h-HSCT either in the absence (-) or presence (+) of 721.221.G target cell lines. One-way ANOVA with Bonferroni correction. ( D ) Summary statistical graph showing the normalized value of PDC1 and LAG3 gene expression in FACS-sorted KIR pos NK cells from NR ( n = 8) and R ( n = 7). Paired t test. ( E ) Pearson correlation between PDCD1 and LAG3 gene expression on KIR pos NK cells. ( F ) Summary statistical graphs showing the percentage of IFN-γ pos NK cells in NR ( n = 8) and R ( n = 8) recipients at 8–12 months after h-HSCT, cocultured with <t>HUVEC</t> either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. ( G ) Summary statistical graphs showing the number of HUVEC cells infected with the BAC clone of the HCMV strain TB40/E expressing EGFP remained after 48 hours of coculture with KIR pos NK cells from NR ( n = 3) and R ( n = 3) either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. * P < 0.05, ** P < 0.01, *** P < 0.001.
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PromoCell growth media
( A ) Gene sets involved in respiratory metabolism and enriched in KIR pos NK cells from NR and retrieved from GO. ( B ) Summary statistical graph showing the percentage of proliferating Ki67 pos /KIR pos NK cells (%, mean ± SD) in NR and R at 7–12 months after h-HSCT. ( C ) Summary statistical graph showing the percentage of KIR pos /NKG2C pos /IFN-γ pos NK cells in NR ( n = 3) and R ( n = 3) recipients at 8–12 months after h-HSCT either in the absence (-) or presence (+) of 721.221.G target cell lines. One-way ANOVA with Bonferroni correction. ( D ) Summary statistical graph showing the normalized value of PDC1 and LAG3 gene expression in FACS-sorted KIR pos NK cells from NR ( n = 8) and R ( n = 7). Paired t test. ( E ) Pearson correlation between PDCD1 and LAG3 gene expression on KIR pos NK cells. ( F ) Summary statistical graphs showing the percentage of IFN-γ pos NK cells in NR ( n = 8) and R ( n = 8) recipients at 8–12 months after h-HSCT, cocultured with <t>HUVEC</t> either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. ( G ) Summary statistical graphs showing the number of HUVEC cells infected with the BAC clone of the HCMV strain TB40/E expressing EGFP remained after 48 hours of coculture with KIR pos NK cells from NR ( n = 3) and R ( n = 3) either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. * P < 0.05, ** P < 0.01, *** P < 0.001.
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PromoCell endothelial basal medium
( A ) Gene sets involved in respiratory metabolism and enriched in KIR pos NK cells from NR and retrieved from GO. ( B ) Summary statistical graph showing the percentage of proliferating Ki67 pos /KIR pos NK cells (%, mean ± SD) in NR and R at 7–12 months after h-HSCT. ( C ) Summary statistical graph showing the percentage of KIR pos /NKG2C pos /IFN-γ pos NK cells in NR ( n = 3) and R ( n = 3) recipients at 8–12 months after h-HSCT either in the absence (-) or presence (+) of 721.221.G target cell lines. One-way ANOVA with Bonferroni correction. ( D ) Summary statistical graph showing the normalized value of PDC1 and LAG3 gene expression in FACS-sorted KIR pos NK cells from NR ( n = 8) and R ( n = 7). Paired t test. ( E ) Pearson correlation between PDCD1 and LAG3 gene expression on KIR pos NK cells. ( F ) Summary statistical graphs showing the percentage of IFN-γ pos NK cells in NR ( n = 8) and R ( n = 8) recipients at 8–12 months after h-HSCT, cocultured with <t>HUVEC</t> either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. ( G ) Summary statistical graphs showing the number of HUVEC cells infected with the BAC clone of the HCMV strain TB40/E expressing EGFP remained after 48 hours of coculture with KIR pos NK cells from NR ( n = 3) and R ( n = 3) either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. * P < 0.05, ** P < 0.01, *** P < 0.001.
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Image Search Results


( A ) Gene sets involved in respiratory metabolism and enriched in KIR pos NK cells from NR and retrieved from GO. ( B ) Summary statistical graph showing the percentage of proliferating Ki67 pos /KIR pos NK cells (%, mean ± SD) in NR and R at 7–12 months after h-HSCT. ( C ) Summary statistical graph showing the percentage of KIR pos /NKG2C pos /IFN-γ pos NK cells in NR ( n = 3) and R ( n = 3) recipients at 8–12 months after h-HSCT either in the absence (-) or presence (+) of 721.221.G target cell lines. One-way ANOVA with Bonferroni correction. ( D ) Summary statistical graph showing the normalized value of PDC1 and LAG3 gene expression in FACS-sorted KIR pos NK cells from NR ( n = 8) and R ( n = 7). Paired t test. ( E ) Pearson correlation between PDCD1 and LAG3 gene expression on KIR pos NK cells. ( F ) Summary statistical graphs showing the percentage of IFN-γ pos NK cells in NR ( n = 8) and R ( n = 8) recipients at 8–12 months after h-HSCT, cocultured with HUVEC either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. ( G ) Summary statistical graphs showing the number of HUVEC cells infected with the BAC clone of the HCMV strain TB40/E expressing EGFP remained after 48 hours of coculture with KIR pos NK cells from NR ( n = 3) and R ( n = 3) either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: JCI Insight

Article Title: Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT

doi: 10.1172/jci.insight.146973

Figure Lengend Snippet: ( A ) Gene sets involved in respiratory metabolism and enriched in KIR pos NK cells from NR and retrieved from GO. ( B ) Summary statistical graph showing the percentage of proliferating Ki67 pos /KIR pos NK cells (%, mean ± SD) in NR and R at 7–12 months after h-HSCT. ( C ) Summary statistical graph showing the percentage of KIR pos /NKG2C pos /IFN-γ pos NK cells in NR ( n = 3) and R ( n = 3) recipients at 8–12 months after h-HSCT either in the absence (-) or presence (+) of 721.221.G target cell lines. One-way ANOVA with Bonferroni correction. ( D ) Summary statistical graph showing the normalized value of PDC1 and LAG3 gene expression in FACS-sorted KIR pos NK cells from NR ( n = 8) and R ( n = 7). Paired t test. ( E ) Pearson correlation between PDCD1 and LAG3 gene expression on KIR pos NK cells. ( F ) Summary statistical graphs showing the percentage of IFN-γ pos NK cells in NR ( n = 8) and R ( n = 8) recipients at 8–12 months after h-HSCT, cocultured with HUVEC either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. ( G ) Summary statistical graphs showing the number of HUVEC cells infected with the BAC clone of the HCMV strain TB40/E expressing EGFP remained after 48 hours of coculture with KIR pos NK cells from NR ( n = 3) and R ( n = 3) either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The EndoGRO HUVECs (MilliporeSigma) were cultured in endothelial cell growth medium-2 (EGM-2) (Lonza) supplemented with 5% FBS, 1% Penicillin-Streptomycin, and 1% Ultra Glutamine in cell flasks precoated with Collagen I (Corning) at a density of 10 5 to 10 6 cells/mL in a humidified atmosphere at 37°C with 5% CO 2 .

Techniques: Gene Expression, Blocking Assay, Infection, Expressing